This video outlines the second step in the process of mushroom cultivation, making an agar medium to more easily propagate a mushroom culture (this step is typically used in large-scale cultivation, if you plan on small-scale self cultivation we recommend inoculating your colonization medium with spores, and skipping to part 4 of the video series).
We use locally sourced Rye Grain (powdered) and Barley Malt extract to form a gelatinous medium which mycelium can colonize. Already colonized grain is required to create a viable agar culture, therefore this method is typically used to propagate mushrooms more quickly (mycelium grows faster on agar than grain), to isolate particularly strong genetics, or as a precursor to making a liquid culture (see part 3). All of this work is done after extensive cleaning, and in front of a flow hood continually blowing filtered air. It is highly recommended that any mycological work is done in front of a flow hood to reduce the chances of contamination later in the mycological life cycle.
Once you have completed the steps shown in this video your agar medium is inoculated and will be ready in 3-4 days to either be cut into chunks and placed in grain bags or made into a liquid culture.
*agar and liquid culture inoculation is typically used for large-scale cultivation, if you plan on small-scale self cultivation a spore print or syringe will be the best (and easiest) option*