This video outlines the third step in the process of mushroom cultivation, making an liquid culture. (this step is typically used in large-scale cultivation, if you plan on small-scale self cultivation we recommend inoculating your colonization medium with spores, and skipping to part 4 of the video series).
We use locally sourced Rye Grain (powdered), Gypsum, Barley Malt extract, and Yeast to form a liquid medium which mycelium can colonize. Already colonized grain or agar is required to create a viable liquid culture, therefore this method is typically used to propagate mushrooms on a large scale, or to isolate particularly strong genetics. All of this work is done after extensive cleaning, and in front of a flow hood continually blowing filtered air. It is highly recommended that any mycological work is done in front of a flow hood to reduce the chances of contamination later in the mycological life cycle.
*agar and liquid culture inoculation is typically used for large-scale cultivation, if you plan on small-scale self cultivation a spore print or syringe will be the best (and easiest) option*